LNP MPs Jon Krause and Tony Perrett have accused the state government of bungling the fire ant control program.
They say a lack of urgency has been shown by the government in treating fire ant nests, with delays up to five months after nests are reported in the Scenic Rim.
But Agriculture Minister Mark Furner has fired back, saying Mr Krause should resign or be sacked for misleading people.
He said the only five-month delay in the district was in aerial treatments because helicopters usually used for fire ants were reassigned to fight catastrophic bushfires in NSW and Victoria.
"The Member for Scenic Rim should seriously consider resigning from his position as an MP or Deb Frecklington should sack him for his shameless attempted exploitation of firefighting efforts to spread false information about the fire ant program," he said.
The independent review did find the program had not always followed protocol "to ensure rigorous management of significant detections".
"One particular instance that came to the attention of this review was the Bromelton infestation where despite immediate initial treatment in February 2019, there was no clearance of the site to ensure RIFA had been removed until the following June," the report said.
"Prompt treatment and follow up in accordance with program protocols are critical..."
Reviews of the 10-year fire ant eradication program were scheduled every two years, with the latest being carried out December by consultant Bernard Wonder, an independent member of the Infrastructure and Regional Development Department's Audit Committee.
Mr Perrett said the review showed the fire ant footprint had expanded and density of larger mounds had increased since the program started in 2017.
"The LNP received complaints about Labor's bungling of the fire ant program right from the start," Mr Perrett said.
"Queenslanders told us they thought it was going backwards, and following the release of the findings, it turned out they were right."
Mr Krause said the report was damning.
"It confirms concerns I have raised since at least 2017," he said.
"Landholders in the Scenic Rim have spoken to me about the lack of urgency shown by the government to actually eradicate fire ants.
"Most notable, a landholder at Peak Crossing reported more than 400 nests on his property and waited eight weeks or longer for them to be treated by a fire ant eradication team.
"Another incident, which anyone can read on page 40 of the review, involved a fire ant infestation at Bromelton initially treated on February 19.
"There was no follow up until five months later."
Mr Furner said reports of fire ants in the region were responded to promptly.
Mr Krause said the review had been commissioned by the Queensland Department of Agriculture and Fisheries.
"The department now needs to act quickly to implement all the recommendations in the report including adopting outcomes-based performance indicators and a review of productivity levels of fire ant eradication teams."
Mr Krause said by the end of this financial year the government would have spent more than $134 million of the $411 million allocated to the program.
"That is a lot of money and tax payers deserve more value for their tax dollars," he said.
"The scheme is clearly not focused on eradicating fire ants, which is the outcome we all want.
"Instead, all I see and the community sees, is the slow southward creep of fire ants."
