A study into the ancestry of dingoes in Australia last year found that the animals have had little interbreeding with domestic dogs.
The dingo, or canis lupus dingo, holds a pure ancestry with as little as 0.6 per cent interbreeding, it was found.
Following the study, the full genetic sequence of a wild-born pure Australian desert dingo, known as Sandy Maliki, was been published in Science Advances.
Led by researchers at La Trobe University, including dingo advocate Professor Bill Ballard, this international collaborative effort has confirmed that pure dingoes are intermediate between wolves (Canis lupus) and domestic dog breeds (Canis lupus familiaris).
Sandy Malaki was discovered in 2014 as a three-week-old pup near the Strzelecki Track in the central Australia desert, along with her sister and brother.
International interest grew when she won the World's Most Interesting Genome competition in 2017.
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Scientists have lined up Sandy's genome against a Greenland wolf (Canis lupus orion), five domestic dog breeds including the German shepherd, and the oldest known dog breed, the Basenji, which originated in Africa.
A previous study looking at single nucleotides of DNA (known as SNPs) placed dingoes as sisters to the Akita and Chow Chow, with the Basenji between this group and the wolf.
However, with the whole genome sequenced, the relationship of the dingo to domestic and ancestral canine lineages can finally be resolved.
From this research, we are also able to gain insight into the desert dingo's biology. For example, the number of copies of a pancreatic 'amylase' gene can assist understanding of its dietary adaptations.
"A pure dingo has only one copy of the amylase gene, whereas domestic dogs have multiple copies - which we show influences the gut microbiome and, we predict, affects what dingoes eat," Professor Ballard said.
"Based on this new knowledge, we hypothesise that dingoes are far less likely to eat farm animals, including sheep.
"If we're correct, what farmers currently assume are dingoes killing their stock, are likely to be feral wild dogs."
This five-year study was a culmination of research undertaken by experts in microbiology, computational biology and veterinary science, from 10 institutions across six countries including Australia, Denmark, Norway, Germany, USA and England, and is a huge step forward in the preservation of this native keystone species.
